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OBJECTIVE@#To explore the mechanism of Pi (Spleen)-deficiency-induced functional diarrhea (FD) model rats treated by Shenling Baizhu Powder (, SBP).@*METHODS@#Thirty male Sprague-Dawley rats were randomly divided into 5 groups including control, model, low-, medium-, and high-dose SBP groups (SBPLDG, SBPMDG, SBPHDG), 6 rats in each group, respectively. Pi-deficiency-induced FD rats model was developed through Radix et Rhizoma Rhei gavage for 7 days. After modeling, the rats were treated with 3 doses of SBP [0.93, 1.86, and 3.72 g/(kg·d)], and the rats in the control and model groups were given pure water for 7 days. The diarrhea index was calculated. On the 7th and 14th days, the traveled distance of rat was measured by the open field test. Serum D-xylose content was determined by the phloroglucinol method and interleukin (IL)-10 and IL-17 levels were measured using an enzyme-linked immunosorbent assay kit. The content of Treg cells was determined by flow cytometry.@*RESULTS@#Compared with the control group, the diarrhea index and IL-17 level in the model group were significantly higher and the total exercise distance and D-xylose content significantly decreased (P>0.05). The expression of IL-10 in the SBPHDG group was significantly up-regulated, and serum D-xylose level and Treg cells increased significantly compared with the model group (P>0.05).@*CONCLUSION@#High-dose SBP exhibited ameliorating effects against Pi-deficiency induced FD, which might be attributed to its modulations on intestinal absorption function as well as adaptive immunity in mesenteric lymph nodes of rat.
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Objective:To investigate the effect of Lycium Barbarum polysaccharides on immune function of erythrocytes in doxorubicin-treated mice.Methods:BALB/c mice were treated with doxorubicin and used as immunosuppression model.The mice were treated with Lycium Barbarum polysaccharides(62.5,125,250 mg/kg) for 7 days,the normal control mice and model control mice were also used in this study.Expression level of CD59 molecule in erythrocytes was analyzed with flow cytometry.The Band-3 level was analyzed by Coomassie Brilliant Blue method.The NKEF-A and NKEF-B expression level in erythrocytes was analyzed by Western blot.The killing activity of NK cells was analyzed with flow cytometry.Results: The level of Band-3,NKEF-A and NKEF-B was decrease in erythrocytes of doxorubicin-treated mice.The killing activity of NK cells was also decrease in the mice when the expression level of CD59 molecule was not change obviously.Lycium Barbarum polysaccharides treatment could promote the recovery of Band-3, NKEF-A and NKEF-B in erythrocytes of the mice.Conclusion:Lycium Barbarum polysaccharides can promote the recovery of immune function of erythrocytes in doxorubicin-treated mice.
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Objective:To investigate the effect of compound polysaccharide with Lentinan,Pachymaran and Tremella polysaccharide on the function of macrophages under various treatment conditions.Methods:The macrophage strain RAW264.7 cells were treated with compound polysaccharide (CP),compound polysaccharide combined with LPS (CP + LPS) and pre-treated with compound polysaccharide and then combined with LPS (Pre-CP + LPS).RAW264.7 cell endocytosis of Rhodamine NP (RNP) was detected by fluorescence microscopy and flow cytometry.The level of NO was detected by Griess reagent.The expression levels of CD40,CD80 and CD86 were detected by flow cytometry.Results:Compound polysaccharides treatment can enhance the phagocytosis of RAW264.7 cells on fluorescent microspheres with time-dependent manner.The compound polysaccharide could promote the secretion of NO in RAW264.7 cells when the concentration was above 1 000 μg/ml.Compound polysaccharide could improve the expression levels of CD40,CD80 and CD86 in RAW264.7 cells.Under the condition of LPS stimulation,both co-treatment and pre-treatment with compound polysaccharide could reduce the expression levels of CD40,CD80 and CD86 in RAW264.7 cells.Conclusion:CP can regulate the immune function of RAW264.7 cells under various treatment conditions that it promotes activation and phagocytosis of the resting state cells and inhibits activation of LPS treated cells.